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Journal of Environment and Biotechnology Research

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Response surface optimization of Cu(II) biosorption onto Candida tropicalis immobilized strontium alginate beads by Box-Behnken experimental design

Journal of Environment and Biotechnology Research, Vol. 8, No. 2, Pages 14-21, Apr 2019

A. Thirunavukkarasu, R. Nithya

DOI: 10.5281/zenodo.2619835


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ABSTRACT

A study was conducted to examine the Cu(II) removal from aqueous solution by immobilized Candida tropicalis in strontium alginate beads using three factor, three-level Box-Behnken experimental design in lab-scale batch study. Prior to the statistical analysis, the Candida tropicalis immobilization was performed using strontium chloride and sodium alginate. A second-order polynomial regression equation was developed to predict Cu(II) removal efficiency as the response using three independent variables, Adsorbent dosage (Cads) ranging from 2.0 to 6.0 g/L, initial pH of the solution (pHo) ranging from 2.0 to 6.0 and initial Cu(II) ion concentration (Co) ranging from 250 to 750 mg/L were coded as x1, x2 and x3 at three levels (-1, 0 and 1). At 95 % confidence limits (α = 0.05), the significance of the independent variables and their interactions were examined using Analysis of Variance (ANOVA). By solving the quadratic regression model, the optimum values of the selected variables (Cads = 6.0 g/L, pHo = 5.24 and Co = 530.05 mg/L) were obtained with the maximum Cu(II) removal efficiency of 98.35 % and the results were validated experimentally.
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Journal of Environment and Biotechnology Research
Volume 8, Number 2, 2019, pp. 14-21
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