Journal of Environment and Biotechnology Research
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Bioaccumulation of heavy metals in steel processing industrial effluents
using Bacillus safensis LAU 13

Journal of Environment & Biotechnology Research, Vol. 6, No. 1, Pages 58-63, January 2017

S.O. Ojoawo, A. Lateef, F.A.Oyeniran, O.T.Kupoluyi, O.S.Opatola, J.O.Daramola



Industrial effluent often contains heavy metals in toxic concentrations which require remediation for
environmental safety purposes. Bioremediation can be used as an effective means of reducing the heavy
concentration in wastewater. This paper focuses on remediation by biosorption as an effective technique for
reduction in the concentration of Cu, Mn, Co, Fe, Zn, Hg, Se, Cd, Pb and Cr present in wastewater samples of a
foundry facility in Ogbomoso and a steel/wire manufacturing industry in Ibadan, Southwest, Nigeria. Initial
concentrations of heavy metals in the wastewater samples after digestion, were determined using atomic
absorption spectrometer (AAS). The glucose enriched wastewater samples were then subjected to bio-treatment
using Bacillus safensis LAU 13 (GenBank accession number KJ461434). The residual pellets after treatment were
subjected to scanning electron microscopy (SEM). At the end of 48 h of growth, biosorption by B. safensis resulted
in 100 % Co remediation and 82.2 - 98.97 % of Mn removal. In steel rolling mill effluent, B. safensis exhibited 97.68 %
Se remediation, while removal ranging between 44 and 55 % were obtained for Cu, Fe and Zn. SEM micrographs
showed the presence of remediated metals in form of aggregates in the bacterial biomass. This study established
the biosorption technique with B. safensis LAU 13 as biosorbent as a viable method in the treatment of industrial
wastewater in terms of material availability, cost-effectiveness and absence of precipitates and slurry.
Journal of Environment and Biotechnology Research
Volume 6, Number 1, 2017, pp. 58-63
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