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Antibacterial activity of the seaweeds Chaetomorpha linum and Padina
gymnospora on human bacterial pathogens

Journal of Environment & Biotechnology Research, Vol. 6, No. 1, Pages 43-52, January 2017

X. Devanya Rosaline, S. Sakthivelkumar, S. Chitra, S. Janarthanan

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ABSTRACT

Seaweeds are important source of bioactive molecules with known beneficial effects on human health. The present
study is designed to investigate the antibacterial efficacy of two chosen marine algae namely Chaetomorpha
linum and Padina gymnospora against some human pathogenic bacteria. Our earlier study on antibacterial
screening using various solvent extracts revealed that the crude acetone extracts of the seaweeds C. linum and
P. gymnospora reacted positively against selected human bacterial pathogens. The crude acetone extracts of
these seaweeds were then fractionated by silica gel column chromatography using sequential gradient of solvent
extraction. It yielded 61 fractions and they were pooled into 9 sub-fractions based on the molecular weight
calculated using thin layer chromatography. The 9 (I to IX) sub-fractions were utilized for their antibacterial
activity against human bacterial pathogens employing disc diffusion method. The sub-fractions I to V of P.
gymnospora were revealed significant antibacterial activity against the tested bacterial strains except,
Methicillin resistant Staphylococcus aureus, Salmonella paratyphii and Klebsiella pneumonia. However, in the
green alga C. linum, the isolated sub-fraction IV responded well to two Gram-positive bacteria (Bacillus subtilis
and Enterococcus faecalis) and the fraction V revealed inhibitory activity against few Gram-negative bacteria
except S. paratyphii, E. coli and Klebsiella pneumonia. The fraction VIII responded to Gram-negative bacteria
except Salmonella paratyphii, Proteus vulgaris and Klebsiella pneumonia. Although there are variations on the
antibacterial activity of different column sub-fractions of these two seaweeds, sub-fractions with significant
activity reported would be useful in isolation of effective antibacterial molecules.
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Journal of Environment and Biotechnology Research
Volume 6, Number 1, 2017, pp. 43-52
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