Journal of Environment and Biotechnology Research
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Studies on acute toxicity of lindane (gamalin 20) exposed to Hetrobrancus
bidorsalis juveniles

Journal of Environment & Biotechnology Research, Vol. 6, No. 1, Pages 117-122, January 2017

James Akaahan Terngu, Tereses Azua Emmanuel, Marry Olabanji Funke



The study was carried out to determine the acute toxicity of lindane (gammalin 20) exposed to Heterobrancus
bidorsalis juveniles using static bioassays. The mean weight and total length of the fish samples were 2.5 g and 8.6
cm, respectively. Six groups of the experimental units were set up containing 10 fish individuals in each bowl with
20 L water capacity. Gammalin 20, organochlorine pesticide was distillated and the active ingredient lindane was
condensed and collected. The stock solution was prepared with graded concentration of 0.02, 0.04, 0.06, 0.08
and 0.10 mL/L. The fish juveniles of 10 individuals were put in each of the bowls and exposed to the different
concentration of the lindane with a control experiment where the toxicant was not introduced. The experimental
set was replicated three times. The exposed fish were observed daily and dead fish were removed immediately and
mortality was recorded for 24 and 48 h exposure period. The LC50 was determined to be 0.06 mL/L for the 24 and
48 h exposure period. The obtained result was transformed to Probit analysis which was plotted against the
graded concentration of lindane for the 24 and 48 h exposure period. The R2 values of 0.76 and 0.80 were
obtained for the 24 and 48 h respectively indicating a strong relationship of lindane with mortality. The result of
the water quality was, pH: 7.80-8.46, temperature: 28.39 - 28.42 0C, DO: 5.06 - 5.17 mg/L, conductivity : 462.00 -
482.00 μS/cm and TDS: 231.00-241.00 mg/L. The water quality parameters increase with increase in the lindane
concentration; however they were within the maximum permissible level and did not have any effect on the fish
and its death. The death of the fish may be due to the toxic potential of lindane. It was recommended through this
study that gammalin 20 is very toxic and persistent in the aquatic environment and its use should be greatly
Journal of Environment and Biotechnology Research
Volume 6, Number 1, 2017, pp. 117-122
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