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Ionic Liquid EMIM acetate induced tailoring of microbially synthesized Au
nanoparticles

Journal of Environment & Biotechnology Research, Vol. 6, No. 1, Pages 1-11, January 2017

Sneha Krishnamurthy, Sok Kim, Chul-Woong Cho, Yeoung-Sang Yun

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ABSTRACT

Though the stability of microbes makes them a potent source for nanoparticle (NP) synthesis, a large percentage
of NPs remain trapped in the biomass after the reaction. This is a major drawback and a protocol needs to be
developed for recovering the NPs from the biomass pellet. The present study focuses on the synthesis of gold NPs
(AuNPs) using marine bacteria Jeotgalibacillus sp., and examines the effects of pH and purification of AuNPs from
the bacterial matrix using ionic liquid (IL) 1-ethyl-3-methylimidazolium (EMIM) acetate. The results demonstrated
that a low concentration of EMIM acetate could be used to extract small NPs (5~50 nm). The use of 50% EMIM
acetate for bacterial treatment facilitated the formation of gold nanoplatelets (150~200 nm) from biogenic Au
seeds. Visual observations and UV-Vis and transmission electron microscopy (TEM) analyses revealed the recovery
of a significant amount of AuNPs. The results demonstrated the successful formation of Au nanoplatelets from
microbially synthesized AuNPs, on treatment with 50% EMIM acetate.
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Journal of Environment and Biotechnology Research
Volume 6, Number 1, 2017, pp. 1-11
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