Journal of Environment and Biotechnology Research

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Evaluation of α-Lactoalbumin and β-Lactoglobulin goat milk whey protein partition by aqueous two-phase systems using polymer/maltodextrin

Journal of Environment and Biotechnology Research, Vol. 8, No. 3, Pages 29-37, July 2019

L.A.C. Freire, C.G. Pereira

DOI: 10.5281/zenodo.3263512



The recovery of biomolecules represents an important field of study in evolution with fundamental application in the advances of biotechnology. In this work, the study of α-lactalbumin (α-La) and β-lactoglobulin (β-Lg) partitioning was carried out using aqueous two-phase systems (formed by polymer/maltodextrin) with different synthetic polymers (polyethylene glycol_PEG, polypropylene glycol_PPG, polyvinylpyrrolidone_PVP) and different molecular weights (from 425 to 40000). The influence of the type, molecular weight and percentage of the polymer used in the system was evaluated in the partition of these proteins. The results indicated that maltodextrin may be a good substitute for dextran in the processes with aqueous two phase aqueous system and it was observed that after the process the proteins tend to remain in the maltodextrin rich phase.

Optimising biogas production through co-digestion of sewage sludge with food waste and FOG

Journal of Environment and Biotechnology Research, Vol. 8, No. 3, Pages 38-51, July 2019

O.W. Awe, R. Liu, Y. Zhao

DOI: 10.5281/zenodo.3263661



This study focused on the assessment of co-digestion of sewage sludge (SS), food waste (FW) and fat, oil and grease (FOG) to optimise biogas production. A series of batch experiments were conducted under mesophilic conditions with different mixtures of SS, FW, and FOG to optimise the best mixing ratio. The performance of the reactors was assessed based on cumulative methane potential, volatile fatty acid (VFA) effects, ammonium (NH4+-N) effects, soluble chemical oxygen demand (SCOD), volatile solids (VS) and, total solids (TS) removal efficiency. The cumulative methane production of mono-digestion of SS, FW, and FOG is 346, 428 and 898 NmL CH4/g VS added, respectively, while the mixture of SS, FW, and FOG with a ratio of 70:25:5 (w/w) could achieve the best methane production of 771 NmL CH4/g VSadded. There was moderate inhibition of VFAs, which could be overcome with a reduction in organic loading rate (OLR) and FW adjustment. NH4+-N was not an inhibition factor in this study. The result also shows a linear increase in methane production and VS reduction in the digestion of the co-mixture of the substrates. Co-digestion can contribute to renewable energy production, diversion of organic waste from landfill and reduction of greenhouse gas (GHG) emissions. Economic and environmental benefits of co-digestion adoption by Ireland wastewater treatment plants (WWTPs) were extensively analyzed and discussed.
Current Issue
Journal of Environment and Biotechnology Research
Volume 8, Number 3, Pages 29-51 (July 2019)

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