Ionic Liquid EMIM acetate induced tailoring of microbially synthesized Au nanoparticles

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Journal of Environment and Biotechnology Research

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Ionic Liquid EMIM acetate induced tailoring of microbially synthesized Au

nanoparticles

Journal of Environment & Biotechnology Research, Vol. 6, No. 1, Pages 1-11, January 2017

Sneha Krishnamurthy, Sok Kim, Chul-Woong Cho, Yeoung-Sang Yun

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ABSTRACT

Though the stability of microbes makes them a potent source for nanoparticle (NP) synthesis, a large percentage

of NPs remain trapped in the biomass after the reaction. This is a major drawback and a protocol needs to be

developed for recovering the NPs from the biomass pellet. The present study focuses on the synthesis of gold NPs

(AuNPs) using marine bacteria Jeotgalibacillus sp., and examines the effects of pH and purification of AuNPs from

the bacterial matrix using ionic liquid (IL) 1-ethyl-3-methylimidazolium (EMIM) acetate. The results demonstrated

that a low concentration of EMIM acetate could be used to extract small NPs (5~50 nm). The use of 50% EMIM

acetate for bacterial treatment facilitated the formation of gold nanoplatelets (150~200 nm) from biogenic Au

seeds. Visual observations and UV-Vis and transmission electron microscopy (TEM) analyses revealed the recovery

of a significant amount of AuNPs. The results demonstrated the successful formation of Au nanoplatelets from

microbially synthesized AuNPs, on treatment with 50% EMIM acetate.

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Journal of Environment and Biotechnology Research

Volume 6, Number 1, 2017, pp. 1-11

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