Experiments were conducted to study the effects of different doses of ovaprim in stimulating ovulation and spawning response of African catfish, Clarias gariepinus. Nine matured female and 9 male fishes weighing from 1.46 to 2.80 kg were randomly selected for three different doses of hormonal injection. Both male and female fishes from all the three replicate groups (3 females and 3 males per replicate) were administered a single dose of 0.4, 0.5 and 0.6 mL of ovaprim/kg body weight (BW), respectively. After 12 h of post-injection, the females were stripped individually into dry and pre weighed plastic containers to record the stripped egg weight per female. The breeding performance was assessed on the basis of total egg mass and stripping response. To assess the egg quality of hormone induced fishes, the fertilization rate and hatching rates were examined among the three doses administered. Complete ovulation was observed in all the three doses of hormonal injection. The results indicated that the total weight of stripped eggs were significantly highest (P < 0.05) at the individuals received 0.4 mL ovaprim/kg BW compared to those injected with other higher doses. Highest fertilization (97.88%) and hatching (93.66%) were also recorded in the individuals received 0.4 mL ovaprim/kg BW. However, breeding performance and egg quality were not statistically significant among the three hormone doses tested in this study. Hence, it is evidenced that ovaprim, the synthetic gonadotropin-releasing hormone with a dopamine antagonist at a dose of 0.4 mL/kg BW could be used as an appropriate spawning dose for C. gariepinus. ______________________________________________________________